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Table 3 Comparison of kinetic parameters of various GAs with maltose and maltotiose as the substrates

From: Glucoamylase of Caulobacter crescentus CB15: cloning and expression in Escherichia coli and functional identification

 

Substrates

Conditions

 

Maltose

Maltotriose

Temp (°C), pH

Enzymes

kcat(s-1)

K m (mM)

kcat/Km(s-1 mM-1)

kcat(s-1)

Km(mM)

kcat/Km(s-1 mM-1)

CauloGA

38.2

0.87

43.9

150

0.16

938

30, 5.0

ClostGA

10.4

3.67

2.83

74.1

0.69

107

25, 4.5

TGA

16.9

0.10

167

76.4

0.38

201

40, 6.5

TtcGA

149

13.4

11.1

368

1.93

191

75, 5.0

AawGA

14.4

1.73

8.3

62

0.73

86

50, 4.4

AmyC

9.3

0.53

17.5

54.7

0.49

112

40, 5.5

  1. The kinetic parameters of various GAs and the conditions for activity measurements are taken from the sources as follows: CauloGA (C. crescentus CB15 glucoamylase), this study; ClostGA (Clostridium; Ohnishi et al. 1992); TGA (Thermoactinomyces vulgaris; Ichikawa et al. 2004); TtcGA (Thermoanaerobacterium tengcongensis; Zheng et al. 2010); AawGA (Aspergillus awamori; Sierks et al. 1989); AmyC (Rhizopus oryzae; Mertens et al. 2010)