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Table 3 Comparison of kinetic parameters of various GAs with maltose and maltotiose as the substrates

From: Glucoamylase of Caulobacter crescentus CB15: cloning and expression in Escherichia coli and functional identification

  Substrates Conditions
  Maltose Maltotriose Temp (°C), pH
Enzymes kcat(s-1) K m (mM) kcat/Km(s-1 mM-1) kcat(s-1) Km(mM) kcat/Km(s-1 mM-1)
CauloGA 38.2 0.87 43.9 150 0.16 938 30, 5.0
ClostGA 10.4 3.67 2.83 74.1 0.69 107 25, 4.5
TGA 16.9 0.10 167 76.4 0.38 201 40, 6.5
TtcGA 149 13.4 11.1 368 1.93 191 75, 5.0
AawGA 14.4 1.73 8.3 62 0.73 86 50, 4.4
AmyC 9.3 0.53 17.5 54.7 0.49 112 40, 5.5
  1. The kinetic parameters of various GAs and the conditions for activity measurements are taken from the sources as follows: CauloGA (C. crescentus CB15 glucoamylase), this study; ClostGA (Clostridium; Ohnishi et al. 1992); TGA (Thermoactinomyces vulgaris; Ichikawa et al. 2004); TtcGA (Thermoanaerobacterium tengcongensis; Zheng et al. 2010); AawGA (Aspergillus awamori; Sierks et al. 1989); AmyC (Rhizopus oryzae; Mertens et al. 2010)