Substrates | kcata(s-1) | Kma(mM) | kcat/Km(s-1mM-1) | Kib(mM) |
---|
Maltose | 38.2 ± 0.94 | 0.87 ± 0.08 | 43.9 | 38.2 ± 1.11 |
Maltotriose | 150 ± 4.37 | 0.16 ± 0.02 | 938 | 4.49 ± 0.31 |
Maltotetraose | 155 ± 0.66 | 0.14 ± 0.004 | 1110 | 1.10 ± 0.06 |
Maltopentaose | 122 ± 4.21 | 0.03 ± 0.008 | 4070 | 0.92 ± 0.04 |
Maltohexaose | 125 ± 1.91 | 0.05 ± 0.004 | 2500 | 1.05 ± 0.08 |
Maltoheptaose | 114 ± 4.57 | 0.04 ± 0.003 | 2850 | 1.25 ± 0.25 |
- akcat and Km values (means ± standard deviation) were obtained based on the results with maltose (0–4.5 mM), maltotriose (0–0.9 mM) and maltotetraose to maltoheptaose (0–0.36 mM) as the substrates. Experiments were performed in triplicate.
- bKi values (means ± standard deviation) were obtained based on the results with maltose to maltotetraose (0–18 mM) and with maltopentaose to maltoheptaose (0–9 mM) as the substrates. Experiments were carried out in triplicate.