|
Substrates
|
kcata(s-1)
|
Kma(mM)
|
kcat/Km(s-1mM-1)
|
Kib(mM)
|
|---|
|
Maltose
|
38.2 ± 0.94
|
0.87 ± 0.08
|
43.9
|
38.2 ± 1.11
|
|
Maltotriose
|
150 ± 4.37
|
0.16 ± 0.02
|
938
|
4.49 ± 0.31
|
|
Maltotetraose
|
155 ± 0.66
|
0.14 ± 0.004
|
1110
|
1.10 ± 0.06
|
|
Maltopentaose
|
122 ± 4.21
|
0.03 ± 0.008
|
4070
|
0.92 ± 0.04
|
|
Maltohexaose
|
125 ± 1.91
|
0.05 ± 0.004
|
2500
|
1.05 ± 0.08
|
|
Maltoheptaose
|
114 ± 4.57
|
0.04 ± 0.003
|
2850
|
1.25 ± 0.25
|
- akcat and Km values (means ± standard deviation) were obtained based on the results with maltose (0–4.5 mM), maltotriose (0–0.9 mM) and maltotetraose to maltoheptaose (0–0.36 mM) as the substrates. Experiments were performed in triplicate.
- bKi values (means ± standard deviation) were obtained based on the results with maltose to maltotetraose (0–18 mM) and with maltopentaose to maltoheptaose (0–9 mM) as the substrates. Experiments were carried out in triplicate.
