Figure 2

Analysis of the CauloGA reaction products with starch (0.45%) (A) and maltotriose (4.5 mM) (B) as the substrates. The reaction was performed at 30°C in 50 mM acetate buffer (pH 4.5), halted by boiling, and the products were analyzed by thin-layer chromatography (TLC) on a Silica gel 60 F₂₅₄ (Merck, Whitehouse Station, NJ, USA) with 1-butanol/ethanol/water (5:5:2, v/v/v) as the solvent, and the carbohydrate spots were detected as chars by spraying the H₂SO₄/methanol (5:95, v/v) solution. G1, G2 and G3 glucose, maltose and maltotriose, respectively. Lane M, glucose standard; lane 1, no enzyme added; lane 2 to 6, the products obtained after hydrolysis for 5 min, 10 min, 15 min, 30 min and 60 min, respectively.