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Figure 4 | AMB Express

Figure 4

From: Degradation of Poly(ε-caprolactone) by thermophilic Streptomyces thermoviolaceus subsp. thermoviolaceus 76T-2

Figure 4

PAGE and zymography of ammonium sulfate precipitate of 76T-2 culture supernatant. Electrophoresis was carried out in a native 8% polyacrylamide gel (A). Zymography was performed by overlaying the gel on another native 8% polyacrylamide gel containing PCL (B 1) or chitin (B 2). The proteins in bands with enzymatic activity were isolated and electrophoresed in a 10% SDS-polyacrylamide gel which was then stained with Coomassie brilliant blue R-250 (C): lane 1, purified 55-kda PCL depolymerase I; lane 2, purified 25-kda PCL depolymerase II.

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