Figure 3
Effect of temperature on PCL degradation by Streptomyces thermoviolaceus subsp. thermoviolaceus isolate 76T-2. 76T-2 cells were cultured at 30°C (─ ○ ─), 40°C (−● -), 45°C (─ ▲ ─), and 50°C (─ □ ─) in basal medium containing 0.3% emulsified PCL powder as the major carbon source. At 2 h intervals, after centrifugation at 12,000Xg for 30 min, the PCL depolymerase activity was assayed by determining the O.D650 value of the culture supernatant.