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Table 2 Nucleotide primers used in this study

From: Enhancing cellulase and hemicellulase production by genetic modification of the carbon catabolite repressor gene, creA, in Acremonium cellulolyticus

Primer Nucleotide sequence
For plasmids construction
creA1000u-f 5′-GGGTCGACTTTACGCAGCTACGCTTAGG-3′
creA1000u-r 5′-CCGAATTCCGAACGGATATTCCTCCAAC-3′
creA1000d-f 5′-CCTCTAGAGGTGACGGGTTTAAATAAGCTG-3′
creA1000d-r 5′-CCGCGGCCGCATGCTACATGCAATCGAGTA-3′
creA2500u-f 5′-GGGTCGACTGACGGAAACGAGAATGCCG-3′
creA2500u-r 5′-GGGAATTCGCGAGGTGTAGTTGGTGTAA-3′
creA2500d-f 5′-CCTCTAGACCTTATTCACGCAACAGCGA-3′
creA2500d-r 5′-CCGCGGCCGCGTCTGGCCGAACACGTGATT-3′
gfp-f 5′-CCGTCGACATGAGTAAAGGAGAAGAACT-3′
gfp-r 5′-GGAATTCATTATTTGTAGAGCTC-3′
creAN-f 5′-CCGTCGACTAACTCCATCACGGAACCGT-3′
creAN-r 5′-CCGGTACCTAACTCCATCACGGAACCGT-3′
For quantitative PCR
Cel5A-f 5′-CACTTGGGGTGTCGACTTCA-3′
Cel5A-r 5′-GGCAAAGGGGATACGGAAAA-3′
Cel5B-f 5′-CGACTCTGACGGGTCTGGTA-3′
Cel5B-r 5′-CTCGCTTTCCGTTGGTTTG-3′
Cel6A-f 5′-GCCGAGATCCCCTCATTTGT-3′
Cel6A-r 5′-CACGGTCAGGCAGGTCATAG-3′
Cel7A-f 5′-GGACTGCCTCCTTCAGCAAA-3′
Cel7A-r 5′-GGCGTAGTCGTCCCACAAAC-3′
Cel7B-f 5′-CCCCGGTACTTCGGTTACTT-3′
Cel7B-r 5′-CGTTGCTGATGTTGTTGTGG-3′
Xyl11B-f 5′-TGCTCTCGGTGTTGATGTTG-3′
Xyl11B-r 5′-GTGGTCTGGTAGTCGGTGGA-3′
Gapdh-f 5′-AACATCATTCCCAGCAGCAC-3′
Gapdh-r 5′-CGGCAGGTCAAGTCAACAAC-3′