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Table 2 Nucleotide primers used in this study

From: Enhancing cellulase and hemicellulase production by genetic modification of the carbon catabolite repressor gene, creA, in Acremonium cellulolyticus

Primer

Nucleotide sequence

For plasmids construction

creA1000u-f

5′-GGGTCGACTTTACGCAGCTACGCTTAGG-3′

creA1000u-r

5′-CCGAATTCCGAACGGATATTCCTCCAAC-3′

creA1000d-f

5′-CCTCTAGAGGTGACGGGTTTAAATAAGCTG-3′

creA1000d-r

5′-CCGCGGCCGCATGCTACATGCAATCGAGTA-3′

creA2500u-f

5′-GGGTCGACTGACGGAAACGAGAATGCCG-3′

creA2500u-r

5′-GGGAATTCGCGAGGTGTAGTTGGTGTAA-3′

creA2500d-f

5′-CCTCTAGACCTTATTCACGCAACAGCGA-3′

creA2500d-r

5′-CCGCGGCCGCGTCTGGCCGAACACGTGATT-3′

gfp-f

5′-CCGTCGACATGAGTAAAGGAGAAGAACT-3′

gfp-r

5′-GGAATTCATTATTTGTAGAGCTC-3′

creAN-f

5′-CCGTCGACTAACTCCATCACGGAACCGT-3′

creAN-r

5′-CCGGTACCTAACTCCATCACGGAACCGT-3′

For quantitative PCR

Cel5A-f

5′-CACTTGGGGTGTCGACTTCA-3′

Cel5A-r

5′-GGCAAAGGGGATACGGAAAA-3′

Cel5B-f

5′-CGACTCTGACGGGTCTGGTA-3′

Cel5B-r

5′-CTCGCTTTCCGTTGGTTTG-3′

Cel6A-f

5′-GCCGAGATCCCCTCATTTGT-3′

Cel6A-r

5′-CACGGTCAGGCAGGTCATAG-3′

Cel7A-f

5′-GGACTGCCTCCTTCAGCAAA-3′

Cel7A-r

5′-GGCGTAGTCGTCCCACAAAC-3′

Cel7B-f

5′-CCCCGGTACTTCGGTTACTT-3′

Cel7B-r

5′-CGTTGCTGATGTTGTTGTGG-3′

Xyl11B-f

5′-TGCTCTCGGTGTTGATGTTG-3′

Xyl11B-r

5′-GTGGTCTGGTAGTCGGTGGA-3′

Gapdh-f

5′-AACATCATTCCCAGCAGCAC-3′

Gapdh-r

5′-CGGCAGGTCAAGTCAACAAC-3′