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Table 1 Strains, plasmids, and primers used in this study

From: Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions

Strain, plasmid or primer

Relevant genotype or description

Reference or source

Strains

  

E. coli

  

  NovaBlue

endA1 hsdR17 (rK12 mK12 +) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+ B+ lacIq Z ΔM15::Tn10] (Tetr)

Merck

  SCS110

dam, dcm supE44, hsdR17, thi leu, rpsL1, lacY, galK, galT, ara, tonA, thr, tsx, Δ(lac-proAB)/F’ [traD36, proAB+ , lacIq , lacZ Δ M15]

Stratagene

C. glutamicum

  

  ATCC13032

Wild-type strain

ATCC

Plasmids

  

  pCC

Kmr; E. coli-C. glutamicum shuttle vector containing cspB promoter

Tateno et al. 2007b

  pCC-pgsA

Kmr; pCC carrying pgsA

This study

  pCC-pgsA-amyA

Kmr; pCC-pgsA carrying amyA gene fused with 3’ terminal of pgsA

This study

Primers

  

  BamHI-pgsA_F

CGCGGATCCATGAAAAAAGAACTGAGCTTTCAT

This study

  pgsA-SacI_R

ACGCGTCGACTTACTTGTCATCGTCATCCTTGTAGTCGAGCTC TTTAGATTTTAGTT TGTCACTATGATC

This study

  SacI-amyA_F

CCCGAGCTCGATGAACAAGTGTCAATGAAAGAT

This study

  amyA-XhoI_R

CCGCTCGAGTTATTTTAGCCCATCTTTATTATAGTTT

This study

  1. ATCC American Type Culture Collection.