Strain, plasmid or primer | Relevant genotype or description | Reference or source |
---|---|---|
Strains | ||
E. coli | ||
NovaBlue | endA1 hsdR17 (rK12 – mK12 +) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+ B+ lacIq Z ΔM15::Tn10] (Tetr) | Merck |
SCS110 | dam, dcm supE44, hsdR17, thi leu, rpsL1, lacY, galK, galT, ara, tonA, thr, tsx, Δ(lac-proAB)/F’ [traD36, proAB+ , lacIq , lacZ Δ M15] | Stratagene |
C. glutamicum | ||
ATCC13032 | Wild-type strain | ATCC |
Plasmids | ||
pCC | Kmr; E. coli-C. glutamicum shuttle vector containing cspB promoter | Tateno et al. 2007b |
pCC-pgsA | Kmr; pCC carrying pgsA | This study |
pCC-pgsA-amyA | Kmr; pCC-pgsA carrying amyA gene fused with 3’ terminal of pgsA | This study |
Primers | ||
BamHI-pgsA_F | CGCGGATCCATGAAAAAAGAACTGAGCTTTCAT | This study |
pgsA-SacI_R | ACGCGTCGACTTACTTGTCATCGTCATCCTTGTAGTCGAGCTC TTTAGATTTTAGTT TGTCACTATGATC | This study |
SacI-amyA_F | CCCGAGCTCGATGAACAAGTGTCAATGAAAGAT | This study |
amyA-XhoI_R | CCGCTCGAGTTATTTTAGCCCATCTTTATTATAGTTT | This study |