Skip to main content

Table 3 Maximum 2,5-DKG reductase activities in cell free extracts of induced and noninduced L. lactis NZ3900 and Lb. plantarum TLG02 cultures

From: Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Not pH regulated cultivations of L. lactis and Lb. plantaruma
Strain /plasmid Volumetric activity (U L -1 fermentation broth) Specific activity (U mg -1 protein) Induction factor b
  Induced Noninduced Induced Noninduced  
L. lactis NZ3900/pVK51dkr 81.6 ± 9.3 16.0 ± 0.24 0.191 ± 0.022 0.038 ± 0.001 5.0
Lb. plantarum/pSIP603dkr 102 ± 5.8 21.7 ± 2.0 0.232 ± 0.022 0.054 ± 0.002 4.3
Lb. plantarum/pSIP609dkr 104 ± 2.75 23.8 ± 2.2 0.243 ± 0.017 0.055 ±0.001 4.4
pH regulated cultivations (pH 6.5) of L. lactis and Lb. plantarum a
Strain /plasmid Volumetric activity (U L -1 fermentation broth) Specific activity (U mg -1 protein) Induction factor b
  Induced Noninduced Induced Noninduced  
L. lactis NZ3900/pVK51dkr 114 ± 1.9 14.6 ± 2.0 0.188 ± 0.001 0.022 ± 0.003 8.5
Lb. plantarum/pSIP603dkr 226 ± 5.9 27.2 ± 0.56 0.264 ± 0.026 0.032 ± 0.001 8.3
Lb. plantarum/pSIP609dkr 262 ± 1.7 30.2 ± 1.2 0.308 ± 0.016 0.033 ± 0.004 9.3
  1. a All data are mean values of three independent experiments. The maximum volumetric and specific activities measured in the culture are presented.
  2. b The induction factor was defined as the ratio between the specific activity obtained under induced conditions and the activity obtained under noninduced conditions.