Not pH regulated cultivations of L. lactis and Lb. plantaruma
|
---|
Strain /plasmid
|
Volumetric activity (U L
-1 fermentation broth)
|
Specific activity (U mg
-1 protein)
|
Induction factor
b
|
---|
|
Induced
|
Noninduced
|
Induced
|
Noninduced
| |
---|
L. lactis NZ3900/pVK51dkr
|
81.6 ± 9.3
|
16.0 ± 0.24
|
0.191 ± 0.022
|
0.038 ± 0.001
|
5.0
|
Lb. plantarum/pSIP603dkr
|
102 ± 5.8
|
21.7 ± 2.0
|
0.232 ± 0.022
|
0.054 ± 0.002
|
4.3
|
Lb. plantarum/pSIP609dkr
|
104 ± 2.75
|
23.8 ± 2.2
|
0.243 ± 0.017
|
0.055 ±0.001
|
4.4
|
pH regulated cultivations (pH 6.5) of
L. lactis
and
Lb. plantarum
a
|
Strain /plasmid
|
Volumetric activity (U L
-1
fermentation broth)
|
Specific activity (U mg
-1
protein)
|
Induction factor
b
|
|
Induced
|
Noninduced
|
Induced
|
Noninduced
| |
L. lactis NZ3900/pVK51dkr
|
114 ± 1.9
|
14.6 ± 2.0
|
0.188 ± 0.001
|
0.022 ± 0.003
|
8.5
|
Lb. plantarum/pSIP603dkr
|
226 ± 5.9
|
27.2 ± 0.56
|
0.264 ± 0.026
|
0.032 ± 0.001
|
8.3
|
Lb. plantarum/pSIP609dkr
|
262 ± 1.7
|
30.2 ± 1.2
|
0.308 ± 0.016
|
0.033 ± 0.004
|
9.3
|
-
a All data are mean values of three independent experiments. The maximum volumetric and specific activities measured in the culture are presented.
-
b The induction factor was defined as the ratio between the specific activity obtained under induced conditions and the activity obtained under noninduced conditions.