Strains or plasmids | Relevant characteristics | Reference or source |
---|---|---|
Strains | Â | Â |
Corynebacterium glutamicum | DMSZ strain 20301 | DMSZ |
Lactobacillus plantarum WCFS1 | a single colony isolated from Lb. plantarum NCIMB8826, which was originally isolated from human saliva (National Collection of Industrial and Marine Bacteria, Aberdeen, U.K.) | Kleerebezem et al. 2003 |
Lactobacillus plantarum TLG02 | WCFS1 derivative, Δalr, D-alanine auxotroph, expression host | Nguyen et al. 2011a |
Lactococcus lactis NZ3900 | NZ3000 derivative, ΔlacF, pepN::nisRK, selection based on the ability to grow on lactose (lacF), expression host | de Ruyter et al. 1996 |
Escherichia coli | Â | Â |
MB2159 | MC1000 derivative, D-alanine auxotroph, cloning host | Strych et al. 2001 |
NEB 5-alpha | cloning host | New England Biolabs |
Plasmids | Â | Â |
pJet1.2/blunt | CloneJET™ PCR Cloning Kit | Fermentas |
NICE derivative plasmids | Â | Â |
pNZ8150 | Cmr, P nisA | |
pTM51R | lac F, pNZ8150 derivative containing Lb. reuteri lacLM genes downstream of P nisA | Maischberger et al. 2010 |
pVK51 | lac F, pTM51R derivative containing the multiple cloning site (from pNZ8150) downstream of P nisA | this work |
pVK51dkr | lac F, pVK51 derivative containing C. glutamicum dkr downstream of P nisA | this work |
pSIP derived plasmids | Â | Â |
pSIP603R | alr, pSIP403 derivative containing Lb. reuteri lacLM controlled by P sppA | Nguyen et al. 2011a |
pSIP609R | alr, pSIP409 derivative containing Lb. reuteri lacLM controlled by P sppQ | Nguyen et al. 2011a |
pSIP603dkr | alr, pSIP603R derivative, lacLM replaced by C. glutamicum dkr controlled by P sppA | this work |
pSIP609dkr | alr, pSIP609R derivative, lacLM replaced by C. glutamicum dkr controlled by P sppQ | this work |