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AMB Express

Table 2 Bacterial strains and plasmids used in this study a

From: Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Strains or plasmids Relevant characteristics Reference or source
Strains   
Corynebacterium glutamicum DMSZ strain 20301 DMSZ
Lactobacillus plantarum WCFS1 a single colony isolated from Lb. plantarum NCIMB8826, which was originally isolated from human saliva (National Collection of Industrial and Marine Bacteria, Aberdeen, U.K.) Kleerebezem et al. 2003
Lactobacillus plantarum TLG02 WCFS1 derivative, Δalr, D-alanine auxotroph, expression host Nguyen et al. 2011a
Lactococcus lactis NZ3900 NZ3000 derivative, ΔlacF, pepN::nisRK, selection based on the ability to grow on lactose (lacF), expression host de Ruyter et al. 1996
Escherichia coli   
MB2159 MC1000 derivative, D-alanine auxotroph, cloning host Strych et al. 2001
NEB 5-alpha cloning host New England Biolabs
Plasmids   
pJet1.2/blunt CloneJET™ PCR Cloning Kit Fermentas
NICE derivative plasmids   
pNZ8150 Cmr, P nisA Mierau and Kleerebezem 2005
pTM51R lac F, pNZ8150 derivative containing Lb. reuteri lacLM genes downstream of P nisA Maischberger et al. 2010
pVK51 lac F, pTM51R derivative containing the multiple cloning site (from pNZ8150) downstream of P nisA this work
pVK51dkr lac F, pVK51 derivative containing C. glutamicum dkr downstream of P nisA this work
pSIP derived plasmids   
pSIP603R alr, pSIP403 derivative containing Lb. reuteri lacLM controlled by P sppA Nguyen et al. 2011a
pSIP609R alr, pSIP409 derivative containing Lb. reuteri lacLM controlled by P sppQ Nguyen et al. 2011a
pSIP603dkr alr, pSIP603R derivative, lacLM replaced by C. glutamicum dkr controlled by P sppA this work
pSIP609dkr alr, pSIP609R derivative, lacLM replaced by C. glutamicum dkr controlled by P sppQ this work
  1. a alr: alanine racemase; Cmr: chloramphenicol resistance; lac F: the gene encoding the soluble carrier enzyme IIA from lactose specific PTS; nisRK: gene necessary for signal transduction integrated in the chromosome; P nisA : promoter nisin A; P sppA , P sppQ : the bacteriocin promoters in the spp gene cluster; lacLM beta-galactosidase encoding gene.
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