Skip to main content

Table 2 Bacterial strains and plasmids used in this study a

From: Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Strains or plasmids

Relevant characteristics

Reference or source

Strains

  

Corynebacterium glutamicum

DMSZ strain 20301

DMSZ

Lactobacillus plantarum WCFS1

a single colony isolated from Lb. plantarum NCIMB8826, which was originally isolated from human saliva (National Collection of Industrial and Marine Bacteria, Aberdeen, U.K.)

Kleerebezem et al. 2003

Lactobacillus plantarum TLG02

WCFS1 derivative, Δalr, D-alanine auxotroph, expression host

Nguyen et al. 2011a

Lactococcus lactis NZ3900

NZ3000 derivative, ΔlacF, pepN::nisRK, selection based on the ability to grow on lactose (lacF), expression host

de Ruyter et al. 1996

Escherichia coli

  

MB2159

MC1000 derivative, D-alanine auxotroph, cloning host

Strych et al. 2001

NEB 5-alpha

cloning host

New England Biolabs

Plasmids

  

pJet1.2/blunt

CloneJET™ PCR Cloning Kit

Fermentas

NICE derivative plasmids

  

pNZ8150

Cmr, P nisA

Mierau and Kleerebezem 2005

pTM51R

lac F, pNZ8150 derivative containing Lb. reuteri lacLM genes downstream of P nisA

Maischberger et al. 2010

pVK51

lac F, pTM51R derivative containing the multiple cloning site (from pNZ8150) downstream of P nisA

this work

pVK51dkr

lac F, pVK51 derivative containing C. glutamicum dkr downstream of P nisA

this work

pSIP derived plasmids

  

pSIP603R

alr, pSIP403 derivative containing Lb. reuteri lacLM controlled by P sppA

Nguyen et al. 2011a

pSIP609R

alr, pSIP409 derivative containing Lb. reuteri lacLM controlled by P sppQ

Nguyen et al. 2011a

pSIP603dkr

alr, pSIP603R derivative, lacLM replaced by C. glutamicum dkr controlled by P sppA

this work

pSIP609dkr

alr, pSIP609R derivative, lacLM replaced by C. glutamicum dkr controlled by P sppQ

this work

  1. a alr: alanine racemase; Cmr: chloramphenicol resistance; lac F: the gene encoding the soluble carrier enzyme IIA from lactose specific PTS; nisRK: gene necessary for signal transduction integrated in the chromosome; P nisA : promoter nisin A; P sppA , P sppQ : the bacteriocin promoters in the spp gene cluster; lacLM beta-galactosidase encoding gene.