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Table 1 Oligonucleotide primers used for PCR amplifications in this study

From: Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Primer name

Restriction enzyme c

Sequence (5′-3′)

Target gene

V1a

-

ATGGATCAGAAGAATAAGCTTTC

dkr

V2b

Spe I

TCTACGACTAGT TCAGTTCAGATCATTCGG

dkr

V3b

Xho I

CTATCGCTCGAG TCAGTTCAGATCATTCGGG

dkr

P1a

Spe I

CGGAAATCACGGGAACTAGT CGCCAAA

P sppA , P sppQ

P2b

-

CGGTACCTACAACAGACATGGGAATCATACTCCTATATATTATT

P sppA

P3b

-

CGGTACCCACAACAGACATATATGCTGGCCAGCTAAGTA

P sppQ

  1. a forward primer.
  2. b reverse primer.
  3. c restriction sites are underlined.