Table 1 Bacterial strains and plasmids used in this study

Strains

E. coli strains

DH5α

Cloning host supE 44 Δlac U169 (Φ 80 lac Z ΔM15) hsdR 17 rec A1 end A1 gyrA 96 thi-1 relA 1

TOYOBO

 S17-1

rec A pro hsdR RP4-2-Tc::Mu-Km::Tn7

Simon et al. (1983)

 S. livingstonensis strains

 Ac10-Rif^r

Parent strain, rifampin resistant mutant of Ac10

Kawamoto et al. (2007)

 Ac10-Rif^r/pHA10-lacZ

Ac10-Rif^r harboring pHA10-lacZ

This study

 Ac10-Rif^r/pHA12-dhaB

Ac10-Rif^r harboring pHA12-dhaB

This study

 K. pneumoniae T7

1,3-propanediol producing strain

Laboratory’s stock

Plasmids

 pHA10

Amp^r, expression vector

Arai et al. (1991)

 pHA12

Amp^r, expression vector

Arai et al. (1991)

 pQF50

Amp^r, broad-host-range vector

Farinha and Kropinski (1990)

 pHA10-lacZ

Amp^r, pHA10 containing lacZ

This study

 pHA12-dhaB

Amp^r, pHA12 containing K. pneumoniae dhaB

This study