TY - JOUR AU - Ikeda, Naoki AU - Miyamoto, Mari AU - Adachi, Noriko AU - Nakano, Mariko AU - Tanaka, Tsutomu AU - Kondo, Akihiko PY - 2013 DA - 2013/11/08 TI - Direct cadaverine production from cellobiose using β-glucosidase displaying Escherichia coli JO - AMB Express SP - 67 VL - 3 IS - 1 AB - In this study, we demonstrate the one-step production of cadaverine (1,5-diaminopentane) from cellobiose using an Escherichia coli strain displaying β-glucosidase (BGL) on its cell surface. L-lysine decarboxylase (CadA) derived from E. coli and BGL from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc from E. coli were co-expressed using E. coli as a host. The expression of CadA was confirmed by Western blotting and BGL activity on the cell surface was evaluated using pNPG as a substrate. Growth on cellobiose as the sole carbon source was also achieved. The OD600 value of the BGL and CadA co-expressing strain was 8.0 after 48 h cultivation, which is higher than that obtained by growth on glucose (5.4 after 48 h cultivation). The engineered strain produced cadaverine from cellobiose more effectively than from glucose: 6.1 mM after 48 h from 28 g/L of consumed cellobiose, vs. 3.3 mM from 20 g/L of consumed glucose. SN - 2191-0855 UR - https://doi.org/10.1186/2191-0855-3-67 DO - 10.1186/2191-0855-3-67 ID - Ikeda2013 ER -