Table 2 Percentage (mean ± S.D.) of E. coli PHL644 pSTB7 cells that were alive determined using flow cytometry during biotransformations performed with planktonic cells or biofilms
From: Optimisation of engineered Escherichia coli biofilms for enzymatic biosynthesis of l-halotryptophans
Reaction conditions | Cell type and time of sampling | |||
|---|---|---|---|---|
Planktonic | Planktonic | Biofilm | Biofilm | |
2 hours | 24 hours | 2 hours | 24 hours | |
Reaction Buffer, 5% DMSO | 99.52 ± 0.14 | 99.32 ± 0.40 | 95.73 ± 2.98 | 92.34 ± 0.10 |
Reaction Buffer, 5% DMSO, 2 mM 5-fluoroindole | 99.38 ± 0.60 | 99.24 ± 0.80 | 96.44 ± 1.51 | 90.73 ± 0.35 |
Reaction Buffer, 5% DMSO, 2 mM 5-chloroindole | 99.27 ± 0.33 | 99.33 ± 0.20 | 95.98 ± 2.64 | 91.69 ± 3.09 |
Reaction Buffer, 5% DMSO, 2 mM 5-bromoindole | 99.50 ± 0.18 | 99.33 ± 0.20 | 96.15 ± 1.94 | 91.17 ± 2.19 |