Table 5 Comparison of Methanomicrobiales percentage obtained by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing
From: Comparative evaluation of rumen metagenome community using qPCR and MG-RAST
Methanomicrobiales (% ± SD) | |||
|---|---|---|---|
Sample | qPCR | Shotgun | |
50% roughage | GL | 0.05 ± 0.0002 | 0.08 ± 0.000 |
DL | 0.38 ± 0.005 | 0.03 ± 0.000 | |
GS | 0.02 ± 0.008 | 0.13 ± 0.0005 | |
DS | 0.01 ± 0.0002 | 0.23 ± 0.003 | |
75% roughage | GL | 0.20 ± 0.001 | 0.15 ± 0.0006 |
DL | 0.60 ± 0.0006 | 0.10 ± 0.000 | |
GS | 0.01 ± 0.000 | 0.09 ± 0.000 | |
DS | 0.002 ± 0.000 | 0.09 ± 0.000 | |
100% roughage | GL | 0.06 ± 0.0005 | 0.07 ± 0.0001 |
DL | 0.16 ± 0.001 | 0.15 ± 0.0006 | |
GS | 0.002 ± 0.000 | 0.09 ± 0.0001 | |
DS | 0.04 ± 0.0004 | 0.10 ± 0.000 | |