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Table 5 Comparison of Methanomicrobiales percentage obtained by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing

From: Comparative evaluation of rumen metagenome community using qPCR and MG-RAST

  Methanomicrobiales (% ± SD)
Sample qPCR Shotgun
50% roughage GL 0.05 ± 0.0002 0.08 ± 0.000
DL 0.38 ± 0.005 0.03 ± 0.000
GS 0.02 ± 0.008 0.13 ± 0.0005
DS 0.01 ± 0.0002 0.23 ± 0.003
75% roughage GL 0.20 ± 0.001 0.15 ± 0.0006
DL 0.60 ± 0.0006 0.10 ± 0.000
GS 0.01 ± 0.000 0.09 ± 0.000
DS 0.002 ± 0.000 0.09 ± 0.000
100% roughage GL 0.06 ± 0.0005 0.07 ± 0.0001
DL 0.16 ± 0.001 0.15 ± 0.0006
GS 0.002 ± 0.000 0.09 ± 0.0001
  DS 0.04 ± 0.0004 0.10 ± 0.000
  1. GL = Green liquid, DL = Dry liquid, GS = Green solid, DS = Dry solid.