Table 4 Comparison of Selenomonas ruminantium percentage by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing
From: Comparative evaluation of rumen metagenome community using qPCR and MG-RAST
Selenomonas ruminantium(% ± SD) | ||||
|---|---|---|---|---|
Sample | qPCR | Shotgun | Amplicon | |
50% roughage | GL | 0.063 ± 0.0003 | 0.003 ± 0.000 | 0.400 ± 0.003 |
DL | 0.040 ± 0.0001 | 0.005 ± 0.000 | 0.430 ± 0.001 | |
GS | 0.006 ± 0.000 | 0.004 ± 0.000 | 0.750 ± 0.002 | |
DS | 0.008 ± 0.000 | 0.003 ± 0.000 | 1.000 ± 0.000 | |
75% roughage | GL | 0.090 ± 0.000 | 0.004 ± 0.000 | 0.800 ± 0.000 |
DL | 0.005 ± 0.000 | 0.002 ± 0.000 | 0.800 ± 0.000 | |
GS | 0.050 ± 0.000 | 0.003 ± 0.000 | 2.000 ± 0.000 | |
DS | 0.001 ± 0.000 | 0.004 ± 0.000 | 1.000 ± 0.000 | |
100% roughage | GL | 0.002 ± 0.001 | 0.004 ± 0.000 | 0.770 ± 0.001 |
DL | 0.002 ± 0.001 | 0.002 ± 0.000 | 0.800 ± 0.0005 | |
GS | 0.005 ± 0.002 | 0.002 ± 0.000 | 2.000 ± 0.007 | |
DS | 0.002 ± 0.0005 | 0.001 ± 0.000 | 1.000 ± 0.001 | |