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Table 4 Comparison of Selenomonas ruminantium percentage by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing

From: Comparative evaluation of rumen metagenome community using qPCR and MG-RAST

  Selenomonas ruminantium(% ± SD)
Sample qPCR Shotgun Amplicon
50% roughage GL 0.063 ± 0.0003 0.003 ± 0.000 0.400 ± 0.003
DL 0.040 ± 0.0001 0.005 ± 0.000 0.430 ± 0.001
GS 0.006 ± 0.000 0.004 ± 0.000 0.750 ± 0.002
DS 0.008 ± 0.000 0.003 ± 0.000 1.000 ± 0.000
75% roughage GL 0.090 ± 0.000 0.004 ± 0.000 0.800 ± 0.000
DL 0.005 ± 0.000 0.002 ± 0.000 0.800 ± 0.000
GS 0.050 ± 0.000 0.003 ± 0.000 2.000 ± 0.000
DS 0.001 ± 0.000 0.004 ± 0.000 1.000 ± 0.000
100% roughage GL 0.002 ± 0.001 0.004 ± 0.000 0.770 ± 0.001
DL 0.002 ± 0.001 0.002 ± 0.000 0.800 ± 0.0005
GS 0.005 ± 0.002 0.002 ± 0.000 2.000 ± 0.007
  DS 0.002 ± 0.0005 0.001 ± 0.000 1.000 ± 0.001
  1. GL = Green liquid, DL = Dry liquid, GS = Green solid, DS = Dry solid.