AMB Express

Table 2 Comparison of Fibrobacter succinogens percentage by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing

From: Comparative evaluation of rumen metagenome community using qPCR and MG-RAST

Sample		*Fibrobacter succinogens*(% ± SD)
Sample		qPCR	Shotgun	Amplicon
50% roughage	GL	0.33 ± 0.005	0.90 ± 0.001	0.33 ± 0.002
	DL	0.35 ± 0.001	0.70 ± 0.000	0.70 ± 0.004
	GS	0.20 ± 0.001	0.43 ± 0.0005	0.35 ± 0.001
	DS	0.11 ± 0.0007	0.38 ± 0.0005	0.37 ± 0.001
75% roughage	GL	0.40 ± 0.003	1.23 ± 0.005	3.00 ± 0.000
	DL	0.38 ± 0.003	1.63 ± 0.011	1.75 ± 0.009
	GS	0.10 ± 0.0004	0.50 ± 0.0008	0.70 ± 0.000
	DS	0.02 ± 0.000	0.40 ± 0.0008	0.28 ± 0.002
100% roughage	GL	0.20 ± 0.002	0.53 ± 0.001	0.55 ± 0.311
	DL	0.78 ± 0.003	2.00 ± 0.000	3.00 ± 0.000
	GS	0.06 ± 0.0005	0.38 ± 0.002	0.18 ± 0.112
	DS	0.01 ± 0.000	0.28 ± 0.0005	0.09 ± 0.020

GL = Green liquid, DL = Dry liquid, GS = Green solid, DS = Dry solid.

Back to article page