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Table 2 Comparison of Fibrobacter succinogens percentage by qPCR and MG-RAST analysis for same samples processed by shotgun and amplicon sequencing

From: Comparative evaluation of rumen metagenome community using qPCR and MG-RAST

Sample Fibrobacter succinogens(% ± SD)
qPCR Shotgun Amplicon
50% roughage GL 0.33 ± 0.005 0.90 ± 0.001 0.33 ± 0.002
DL 0.35 ± 0.001 0.70 ± 0.000 0.70 ± 0.004
GS 0.20 ± 0.001 0.43 ± 0.0005 0.35 ± 0.001
DS 0.11 ± 0.0007 0.38 ± 0.0005 0.37 ± 0.001
75% roughage GL 0.40 ± 0.003 1.23 ± 0.005 3.00 ± 0.000
DL 0.38 ± 0.003 1.63 ± 0.011 1.75 ± 0.009
GS 0.10 ± 0.0004 0.50 ± 0.0008 0.70 ± 0.000
DS 0.02 ± 0.000 0.40 ± 0.0008 0.28 ± 0.002
100% roughage GL 0.20 ± 0.002 0.53 ± 0.001 0.55 ± 0.311
DL 0.78 ± 0.003 2.00 ± 0.000 3.00 ± 0.000
GS 0.06 ± 0.0005 0.38 ± 0.002 0.18 ± 0.112
  DS 0.01 ± 0.000 0.28 ± 0.0005 0.09 ± 0.020
  1. GL = Green liquid, DL = Dry liquid, GS = Green solid, DS = Dry solid.