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AMB Express
Figure 2 | AMB Express

Figure 2

From: Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE

Figure 2

Characterization of merE in transgenic plant lines. The structure of the DNA region transferred using the plant expression plasmids pMAE2 (A). Confirmation of the expression of merE (E2, E3, E4, E5, E6, and E7) in transgenic plants based on genomic PCR expression analysis (B). Expression analyses of merE (E2, E3, E4, E5, E6, and E7) in transgenic plants, which were determined by reverse transcription PCR (C). Transgenic plants were grown on MS gellan gum plates. Plates were incubated at 22°C for 2 weeks. Genome DNA prepared from transgenic plants and used for genomic PCR analysis of merE (U-Not-merE and L-Xba-merE) and NPT II (U-321NPTII and L-1109NPTII). The total RNA was prepared from transgenic plants and used for reverse transcription PCR analysis to determine the merE (U-Not-merE and L-Xba-merE) and Actin (primer β-ACT-Fd and β-ACT-Rv) transcription levels.

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