Cultivation of P. putida KT2440 by feeding nonanoic acid, glucose and acrylic acid at a mass ratio of 1.25: 1: 0.01 and a specific growth rate of 0.25 h-1
in fed-batch fermentation at 28.5 ± 1°C, pH 6.85 ± 0.05, DO > 30%. Fermentation data (a) and (b), polymer composition (c). Foam was controlled by manual injection of antifoam 204 in 1% (v/v) nonanoic acid as required. HN,3-hydroxynonanoate; HHp, 3-hydroxyheptanoate. Symbols are the average of two samples and error bars represent the range of the average.