Benzene oxygenation and oxidation by the peroxygenase of Agrocybe aegerita

AMB Express

Table 1 Origin of oxygen in the hydroxylation and oxidation products of benzene and phenol formed by Aae APO in the presence of H ₂ ¹⁸ O ₂

Substrate	Oxygen atoms in hydroquinone [rel.%]			Oxygen atoms in p-benzoquinone [rel.%]			Oxygen atoms in catechol [rel.%]
	¹⁶ O/¹⁶ O	¹⁶ O/¹⁸ O	¹⁸ O/¹⁸ O	¹⁶ O/¹⁶ O	¹⁶ O/¹⁸ O	¹⁸ O/¹⁸ O	¹⁶ O/¹⁶ O	¹⁶ O/¹⁸ O	¹⁸ O/¹⁸ O
Benzene	n.d.^§	n.d.^§	n.d.^§	20	55	25	100	0	0
Benzene^#	9	25	66	n.d.^{§ §}	n.d.^{§ §}	n.d.^{§ §}	18	31	51
Phenol	42	58	0	42	58	0	100	0	0
Phenol^#	3	97	0	n.d.^{§ §}	n.d.^{§ §}	n.d^§§	6	94	0

^# in presence of ascorbic acid (4 mM).
^§ n.d. – not detectable, the concentration was too low to give a viable LC-MS signal.
^§§ n.d. – not detected in the presence of ascorbic acid.