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Table 4 Effect of organic solvents on the activity of laccase from T. fusca BCRC 19214

From: Properties of the newly isolated extracellular thermo-alkali-stable laccase from thermophilic actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation

Organic compounds

Concentration (%, v/v)

Relative activity* (%)

None

-

100

Methanol

10

59 ± 3

 

20

34 ± 3

Ethanol

10

66 ± 3

 

20

40 ± 4

Isopropanol

10

61 ± 2

 

20

36 ± 4

Acetone

10

50 ± 2

 

20

20 ± 1

Dimethyl sulfoxide

10

101 ± 1

(DMSO)

20

74 ± 4

Acetonitrile

10

160 ± 6

 

20

118 ± 5

Dimethylformamide

10

120 ± 4

(DMF)

20

110 ± 5

  1. * The relative activity was measured at 50°C for 15 min, using 20 mM 2,6- dimethoxyphenol (2,6-DMP) as the substrate in 20 mM phosphate buffer (pH 8.0).