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Table 3 Effect of inhibitors on the activity of laccase from T. fusca BCRC 19214

From: Properties of the newly isolated extracellular thermo-alkali-stable laccase from thermophilic actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation

Inhibitor

Concentration (mM)

Relative activity* (%)

None

-

100

SDS

0.1

94 ± 3

 

1

73 ± 3

 

10

63 ± 6

sodium azide

0.1

87 ± 2

 

1

84 ± 4

 

10

80 ± 3

⋅-mercaptoethanol

0.1

86 ± 3

 

1

4 ± 2

 

10

0 ± 1

EDTA

0.1

24 ± 2

 

1

23 ± 2

 

10

5 ± 1

  1. * The relative activity was measured at 50°C for 15 min, using 20 mM 2,6- dimethoxyphenol (2,6-DMP) as the substrate in 20 mM phosphate buffer (pH 8.0). SDS, Sodium dodecyl sulfate; EDTA, Ethylenediaminetetraacetic acid.
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