Table 1 Summary of the purification of laccase from T. fusca BCRC 19214
Step | Volume (mL) | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|---|
Crude culture filtrate | 760.00 | 12.31 | 49.04 | 3.98 | 1.00 | 100.00 |
Ultrafiltration | 20.00 | 7.94 | 39.09 | 11.27 | 2.83 | 79.71 |
Q-Sepharose FF | 16.00 | 2.36 | 30.75 | 13.05 | 3.28 | 62.71 |
Sephacryl™ S-200 | 6.00 | 0.20 | 3.68 | 18.50 | 4.64 | 7.49 |