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Figure 5 | AMB Express

Figure 5

From: Properties of the newly isolated extracellular thermo-alkali-stable laccase from thermophilic actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation

Figure 5

Effects of temperature and pH on stability and activity of purified laccase from T. fusca BCRC 19214. (a), the activity was measured at various temperatures 40-80°C in pH 8.0 for 15 min. (b), the residual activity, measured with 2,6-DMP at 50°C, was determined after the enzyme had been incubated at 50-70°C in pH 8.0 for 0–240 min. (c), the activity was measured in various buffer solutions, pH 5.0-10.0, at 50°C. Glycine-NaOH buffer (20 mM, pH 10.0), Tris–HCl buffer (20 mM, pH 8.0-9.0), phosphate buffer (20 mM, pH 6.0-7.0), and citric acid-citrate buffer (20 mM, pH 5.0) were used. (d), the residual activity, measured at 50°C, was determined after the enzyme had been incubated in various buffer solutions, pH 6–10, at 4°C for 24 h. These data are representative of three replicate experiments.

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