Table 1 Sequences of primers used in this study
Name | Primer sequence a(from 5′ end to 3' end) | Restriction site |
|---|---|---|
Primers for ABF-2 gene | F = GGAATTCCATATG GACATCG | Nde І |
ACTTTAGTACTTGTGC | ||
R = CCGCTCGAG TTATCCTCTCT | Xho І | |
TAATAAGAGCACCAAG | ||
Primers for HLA gene | F = GAATTCTCATGA AGCAATTC | Bsp HІ |
ACAAAATGTGAGCTG | ||
R = CGGGATCC TTACAACTTCTC | Bam HІ | |
ACAAAGCCACTG | ||
Primers for BLA gene | F = GAATTCCCATGG AACAGTTA | Nco І |
ACAAAATGTGAGGTG | ||
R = CGGGATCC TTACAACTTCTC | Bam HІ | |
ACAGAGCCA | ||
Primers for HLZ gene | F = GAATTCTCATGA AGGTCTTT | Bsp HІ |
GAAAGGTGTGAGTTG | ||
R = CGGGATCC TTACACTCCACA | Bam HІ | |
ACCTTGAACATAC | ||
Primers for BLZ gene | F = GAATTCTCATGA AGGTCTTT | Bsp HІ |
GAGAGATGTGAGC | ||
R = CGGGATCC TTACAGGGTGCA | Bam HІ | |
ACCCTCAA |