Table 1 Oligonucleotides used during the study
From: Characterization of erythrose reductases from filamentous fungi
Name | Sequence (5′ - 3′) a | Usage |
|---|---|---|
err1_A.nig_BamHI_f | ATATAGGATCC ATGTCTCTCGGAAAGAAGGTTACTCTC | pGEX-err1A |
err1_A.nig_NotI_r | TATATGCGGCCGC TTAAACAATCACCTTATGACCAGCAGGC | pGEX-err1A |
err1_T.ree_BamHI_f | ATATAGGATCC ATGTCTTCCGGAAGGACC | pGEX-err1T |
err1_T.ree_NotI_r | TATATGCGGCCGC TTACAGCTTGATGACAGCAGTG | pGEX-err1T |
err1_F.gra_BamHI_f | ATATAGGATCC ATGTCTTTCGGTCGAACTGTCACTC | pGEX-err1F |
err1_F.gra_NotI_r | TATATGCGGCCGC TTACAGCTTGAGAACAACCTGGTGG | pGEX-err1F |
err1_XbaI_f | ATATATCTAGA ATGTCTTCCGGAAGGACC | Vector construction for fungal transformation |
err1_Nsi_r | TATATATGCAT TTACAGCTTGATGACAGCAGTG | |
qerr1_f | CTTTACCATTGAGCACCTCGACG | RT-qPCR err1 |
qerr1_r | GGTCTTGCCCTGCTTCTTGG | RT-qPCR err1 |
qact1_f | TGAGAGCGGTGGTATCCACG | RT-qPCR act1 |
qact1_r | GGTACCACCAGACATGACAATGTTG | RT-qPCR act1 |
qsar1_f | TGGATCGTCAACTGGTTCTACGA | RT-qPCR sar1 |
qsar1_r | GCATGTGTAGCAACGTGGTCTTT | RT-qPCR sar1 |