Figure 3

Influence of err1 overexpression in T. reesei in vivo. Strains were grown in duplicates on MA medium supplemented with 1% D-xylose in shakeflasks. Samples of mycelia were drawn after 24 h and 30 h. (a) Relative transcript levels of err1 in the T. reesei parental strain (light grey) and the err1 overexpression strain (dark grey). Transcript levels of err1 were analysed in triplicates by RT-qPCR and normalized using sar1 and act1 as reference genes. All transcript levels refer to the one from the parental strain after 24 h (reference sample). (b) Erythritol formation in the T. reesei parental strain (light grey) and the err1 overexpression strain (dark grey). Erythritol content of the mycelia was determined in triplicates by GC analysis. Error bars indicate standard deviations.