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Figure 1 | AMB Express

Figure 1

From: Engineered Pichia pastoris for enhanced production of S-adenosylmethionine

Figure 1

Confirmation of transformed clones of P. pastoris by colony PCR with AOX and gene specific primers. a: Amplification with AOX primers. Lanes 1, 2, 3, 6, 7 and 8: Clones exhibiting an amplified thick band of ~ 1.4 Kb SAM2 gene and a faint band of 2.2 Kb AOX gene. Lane 4: Untransformed colony exhibiting amplified band of 2.2 Kb AOX gene. Lane 5: DNA Marker (1.0 Kb ladder) showing bands of 250 bp, 500 bp, 750 bp, 1.0 Kb, 1.5 Kb, 2.0 Kb &2.5 Kb. b: Amplification with gene specific primers of SAM2 gene. Lane 1: DNA Marker (1.0 Kb ladder) showing bands of 750 bp, 1 Kb, 1.5 Kb, 2.0 Kb, 2,5 Kb, 3.0 Kb, 3.5 Kb, 4.0 Kb, 5.0 Kb and 6.0 Kb. Lanes 2, 3, 4, 6, 7 and 8: Clones exhibiting amplified band of ~ 1.2 Kb corresponds to SAM2 gene. Lane 5: Untransformed GS115 strain showing no amplification.

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