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Table 1 List of primers used in this study

From: Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus

Primers used to clone the Aaku80 segment
ku80F1 5’-ATGGCNGANAAGGAAGCAACNGT-3’
ku80R3 5’-CGTCCATAYTCATANCCYTTGGC-3’
Primers used to clone the AaargB segment
AaargBF3 5’-CARGCNATHGCNGAYTTYCARAC-3’
AaargBR2 5’-GGNARRCARTGCATRAAYYTCCA-3’
Primers used for the Aaku80 deletion
aku80AF1 5’-GCGGCCGCATGGTTCTATGTCGCTTGGC-3’
aku80AR2 5’-CGACTGGGTCTCTGCGATGGCGAGTAGCGACGAAG-3’
aku80BF1 5’-TGTTTATTGCAGCCAGCATGATGGTGCCAACGCAG-3’
aku80BR1 5’-GCGGCCGCATTGCGATGCTGGGTGAC-3’
aku80CF1 5’-CTTCGTCGCTACTCGCCATCGCAGAGACCCAGTCG-3’
aku80CR1 5’-GAGAGCATTGTCTGCGGATGGTGGGGATGGGTTG-3’
npyrF2 5’-CAACCCATCCCCACCATCCGCAGACAATGCTCTC-3’
npyrR1 5’-CTGCGTTGGCACCATCATGCTGGCTGCAATAAACA-3’
Primers used for the A. nidulans pyrG insertion into Aaku80
coku80NF 5’-TAGATATC AGCTCTTCTTGCCATCGC-3’
coku80NR 5’-TTAGCGGCCGC TGAAACCATCAGTCTTGTCTGC-3’
coku80CF 5’-GCCTTAATTAA GAGTCCCACGAAGCGATG-3’
coku80CR 5’-TGCTCTAGA TGTCGTCATCTTGATAGCGA-3’
copyrF 5’-TAGCGGCCGC AATGCTCTCTATC-3’
copyrR 5’-CCTTAATTAA CCGTTACACATTTCCACTCA-3’
Primers used for the AaargB disruption
AaargB5F 5’-GGCGTGATTCTTTGCTCC-3’
AaargB5r2 5’-GAGAGCATTGTCTGCGGGGTCGGCGTCAGGTC-3’
AaargB3f3 5’-GAGTGGAAATGTGTAACGGATTGCATGGACCCGAG-3’
AaargB3r 5’-GGGGACATGGCTTCCTAC-3’
npyrF3 5’-GACCTGACGCCGACCCCGCAGACAATGCTCTC-3’
npyrR3 5’-CTCGGGTCCATGCAATATGCTGGCTGCAATAAAC-3’
mrarg5F 5’-GGCGGGCTGGAGCTCAC-3’
mrarg5iR 5’-GCACCGCGCTAGCCGGTCATTCG-3’
mrarg5iF 5’-CGAATGACCGGATAGCGCGGTGC-3’
mrarg5R 5’-GAGAGCATTGTCTGCGGGCGATCTTGAGGCCTTCCAG-3’
mrpyr5F 5’-CTGGAAGGCCTCAAGATCGCCCGCAGACAATGCTCTC-3’
mrpyr5R 5’-GACAAAATCCTCGCTCTCCTC-3’
mrarg3F1 5’-CGGTATTGACTAAAAGGGATCTGGAAGGCCTCAAGATCGC-3’
mrarg3F2 5’-CGGTATTGACTAAAAGGGATCCGCTGCAGGCCATC-3’
mrarg3F3 5’-CGGTATTGACTAAAAGGGATGCGCCATCTGCTCTCG-3’
mrarg3R 5’-GAAGGTCAAGGCCGGTG-3’
mrpyr3F 5’-TGCCCCTCCAGGATAAC-3’
mrpyr3R1 5’-GCGATCTTGAGGCCTTCCAGATCCCTTTTAGTCAATACCG-3’
mrpyr3R2 5’-GATGGCCTGCAGCGGATCCCTTTAGTCAATACCG-3’
mrpyr3R3 5’-CGAGAGCAGATGGCGCATCCCTTTTAGTCAATACCG-3’
Primers used to amplify DNA probes for Southern blot analyses
ku80R4 5’-GGAACCCAGAAGAATACGACAC-3’
AaargB3f3 5’-GAGTGGAAATGTGTAACGGATTGCATGGACCCGAG-3’
AaargB3r 5’-GGGGACATGGCTTCCTAC-3’
DxlnR-F1 5’-GTCGACCACCGCGATCCAGCAGTACGCCA-3’
DxlnR-R2 5’-CACAATCCACAGTGAGACCCACTCTCTCGC-3’