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Table 1 List of primers used in this study

From: Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus

Primers used to clone the Aaku80 segment

ku80F1

5’-ATGGCNGANAAGGAAGCAACNGT-3’

ku80R3

5’-CGTCCATAYTCATANCCYTTGGC-3’

Primers used to clone the AaargB segment

AaargBF3

5’-CARGCNATHGCNGAYTTYCARAC-3’

AaargBR2

5’-GGNARRCARTGCATRAAYYTCCA-3’

Primers used for the Aaku80 deletion

aku80AF1

5’-GCGGCCGCATGGTTCTATGTCGCTTGGC-3’

aku80AR2

5’-CGACTGGGTCTCTGCGATGGCGAGTAGCGACGAAG-3’

aku80BF1

5’-TGTTTATTGCAGCCAGCATGATGGTGCCAACGCAG-3’

aku80BR1

5’-GCGGCCGCATTGCGATGCTGGGTGAC-3’

aku80CF1

5’-CTTCGTCGCTACTCGCCATCGCAGAGACCCAGTCG-3’

aku80CR1

5’-GAGAGCATTGTCTGCGGATGGTGGGGATGGGTTG-3’

npyrF2

5’-CAACCCATCCCCACCATCCGCAGACAATGCTCTC-3’

npyrR1

5’-CTGCGTTGGCACCATCATGCTGGCTGCAATAAACA-3’

Primers used for the A. nidulans pyrG insertion into Aaku80

coku80NF

5’-TAGATATC AGCTCTTCTTGCCATCGC-3’

coku80NR

5’-TTAGCGGCCGC TGAAACCATCAGTCTTGTCTGC-3’

coku80CF

5’-GCCTTAATTAA GAGTCCCACGAAGCGATG-3’

coku80CR

5’-TGCTCTAGA TGTCGTCATCTTGATAGCGA-3’

copyrF

5’-TAGCGGCCGC AATGCTCTCTATC-3’

copyrR

5’-CCTTAATTAA CCGTTACACATTTCCACTCA-3’

Primers used for the AaargB disruption

AaargB5F

5’-GGCGTGATTCTTTGCTCC-3’

AaargB5r2

5’-GAGAGCATTGTCTGCGGGGTCGGCGTCAGGTC-3’

AaargB3f3

5’-GAGTGGAAATGTGTAACGGATTGCATGGACCCGAG-3’

AaargB3r

5’-GGGGACATGGCTTCCTAC-3’

npyrF3

5’-GACCTGACGCCGACCCCGCAGACAATGCTCTC-3’

npyrR3

5’-CTCGGGTCCATGCAATATGCTGGCTGCAATAAAC-3’

mrarg5F

5’-GGCGGGCTGGAGCTCAC-3’

mrarg5iR

5’-GCACCGCGCTAGCCGGTCATTCG-3’

mrarg5iF

5’-CGAATGACCGGATAGCGCGGTGC-3’

mrarg5R

5’-GAGAGCATTGTCTGCGGGCGATCTTGAGGCCTTCCAG-3’

mrpyr5F

5’-CTGGAAGGCCTCAAGATCGCCCGCAGACAATGCTCTC-3’

mrpyr5R

5’-GACAAAATCCTCGCTCTCCTC-3’

mrarg3F1

5’-CGGTATTGACTAAAAGGGATCTGGAAGGCCTCAAGATCGC-3’

mrarg3F2

5’-CGGTATTGACTAAAAGGGATCCGCTGCAGGCCATC-3’

mrarg3F3

5’-CGGTATTGACTAAAAGGGATGCGCCATCTGCTCTCG-3’

mrarg3R

5’-GAAGGTCAAGGCCGGTG-3’

mrpyr3F

5’-TGCCCCTCCAGGATAAC-3’

mrpyr3R1

5’-GCGATCTTGAGGCCTTCCAGATCCCTTTTAGTCAATACCG-3’

mrpyr3R2

5’-GATGGCCTGCAGCGGATCCCTTTAGTCAATACCG-3’

mrpyr3R3

5’-CGAGAGCAGATGGCGCATCCCTTTTAGTCAATACCG-3’

Primers used to amplify DNA probes for Southern blot analyses

ku80R4

5’-GGAACCCAGAAGAATACGACAC-3’

AaargB3f3

5’-GAGTGGAAATGTGTAACGGATTGCATGGACCCGAG-3’

AaargB3r

5’-GGGGACATGGCTTCCTAC-3’

DxlnR-F1

5’-GTCGACCACCGCGATCCAGCAGTACGCCA-3’

DxlnR-R2

5’-CACAATCCACAGTGAGACCCACTCTCTCGC-3’