From: Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus
Primers used to clone the Aaku80 segment | |
ku80F1 | 5’-ATGGCNGANAAGGAAGCAACNGT-3’ |
ku80R3 | 5’-CGTCCATAYTCATANCCYTTGGC-3’ |
Primers used to clone the AaargB segment | |
AaargBF3 | 5’-CARGCNATHGCNGAYTTYCARAC-3’ |
AaargBR2 | 5’-GGNARRCARTGCATRAAYYTCCA-3’ |
Primers used for the Aaku80 deletion | |
aku80AF1 | 5’-GCGGCCGCATGGTTCTATGTCGCTTGGC-3’ |
aku80AR2 | 5’-CGACTGGGTCTCTGCGATGGCGAGTAGCGACGAAG-3’ |
aku80BF1 | 5’-TGTTTATTGCAGCCAGCATGATGGTGCCAACGCAG-3’ |
aku80BR1 | 5’-GCGGCCGCATTGCGATGCTGGGTGAC-3’ |
aku80CF1 | 5’-CTTCGTCGCTACTCGCCATCGCAGAGACCCAGTCG-3’ |
aku80CR1 | 5’-GAGAGCATTGTCTGCGGATGGTGGGGATGGGTTG-3’ |
npyrF2 | 5’-CAACCCATCCCCACCATCCGCAGACAATGCTCTC-3’ |
npyrR1 | 5’-CTGCGTTGGCACCATCATGCTGGCTGCAATAAACA-3’ |
Primers used for the A. nidulans pyrG insertion into Aaku80 | |
coku80NF | 5’-TAGATATC AGCTCTTCTTGCCATCGC-3’ |
coku80NR | 5’-TTAGCGGCCGC TGAAACCATCAGTCTTGTCTGC-3’ |
coku80CF | 5’-GCCTTAATTAA GAGTCCCACGAAGCGATG-3’ |
coku80CR | 5’-TGCTCTAGA TGTCGTCATCTTGATAGCGA-3’ |
copyrF | 5’-TAGCGGCCGC AATGCTCTCTATC-3’ |
copyrR | 5’-CCTTAATTAA CCGTTACACATTTCCACTCA-3’ |
Primers used for the AaargB disruption | |
AaargB5F | 5’-GGCGTGATTCTTTGCTCC-3’ |
AaargB5r2 | 5’-GAGAGCATTGTCTGCGGGGTCGGCGTCAGGTC-3’ |
AaargB3f3 | 5’-GAGTGGAAATGTGTAACGGATTGCATGGACCCGAG-3’ |
AaargB3r | 5’-GGGGACATGGCTTCCTAC-3’ |
npyrF3 | 5’-GACCTGACGCCGACCCCGCAGACAATGCTCTC-3’ |
npyrR3 | 5’-CTCGGGTCCATGCAATATGCTGGCTGCAATAAAC-3’ |
mrarg5F | 5’-GGCGGGCTGGAGCTCAC-3’ |
mrarg5iR | 5’-GCACCGCGCTAGCCGGTCATTCG-3’ |
mrarg5iF | 5’-CGAATGACCGGATAGCGCGGTGC-3’ |
mrarg5R | 5’-GAGAGCATTGTCTGCGGGCGATCTTGAGGCCTTCCAG-3’ |
mrpyr5F | 5’-CTGGAAGGCCTCAAGATCGCCCGCAGACAATGCTCTC-3’ |
mrpyr5R | 5’-GACAAAATCCTCGCTCTCCTC-3’ |
mrarg3F1 | 5’-CGGTATTGACTAAAAGGGATCTGGAAGGCCTCAAGATCGC-3’ |
mrarg3F2 | 5’-CGGTATTGACTAAAAGGGATCCGCTGCAGGCCATC-3’ |
mrarg3F3 | 5’-CGGTATTGACTAAAAGGGATGCGCCATCTGCTCTCG-3’ |
mrarg3R | 5’-GAAGGTCAAGGCCGGTG-3’ |
mrpyr3F | 5’-TGCCCCTCCAGGATAAC-3’ |
mrpyr3R1 | 5’-GCGATCTTGAGGCCTTCCAGATCCCTTTTAGTCAATACCG-3’ |
mrpyr3R2 | 5’-GATGGCCTGCAGCGGATCCCTTTAGTCAATACCG-3’ |
mrpyr3R3 | 5’-CGAGAGCAGATGGCGCATCCCTTTTAGTCAATACCG-3’ |
Primers used to amplify DNA probes for Southern blot analyses | |
ku80R4 | 5’-GGAACCCAGAAGAATACGACAC-3’ |
AaargB3f3 | 5’-GAGTGGAAATGTGTAACGGATTGCATGGACCCGAG-3’ |
AaargB3r | 5’-GGGGACATGGCTTCCTAC-3’ |
DxlnR-F1 | 5’-GTCGACCACCGCGATCCAGCAGTACGCCA-3’ |
DxlnR-R2 | 5’-CACAATCCACAGTGAGACCCACTCTCTCGC-3’ |