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Table 2 Sequencing results of vector backbones that were subjected to epMEGAWHOP optimization

From: Increasing protein production by directed vector backbone evolution

pET28a(+)-CelA2 pET22b(+)-BSLA pHY-scarlsberg
bp Substitution Region bp Substitution Region bp Substitution Region
  wt - M1    wt - M1    wt - M1  
488 A - T f1 origin 123 T - C T7 term 209 A - G Tet
850 G - C Kan 2007 A - G na 684 A - G Tet
2233 T - C pBR322 ori 3701 A - G Tet 1584 T - C pTet
2396 T - C na 4563 T - C lacI 3238 T - C pAMP
3170 T - C na 4794 C - T lacI 4221 T - C na
5193 A - T T7 prom       4332 A - - ori-177
  1. The positions of the mutations in the corresponding vector backbones are based on the sequenced vector backbone prior epMEGAWHOP. Alignments of all three vector systems to the corresponding ‘parents’ are included in Additional file 1: Figure S1. Nucleotide position 1 is the base after the stop codon (ATT, TAA) of the inserted genes.
  2. na: no corresponding function of the gene sequence could be assigned.
  3. Kan/Tet: Kanamycin/Tetracyclin resistance gene.
  4. pTET/pAMP: promoter region Tetracyclin/Ampicilin resistance gene (identified with a promoter prediction tool (Reese 2001)).