From: Increasing protein production by directed vector backbone evolution
Expression host | Plasmid | Enzyme | Location of expressed enzyme |
---|---|---|---|
E. coli BL21-Gold (DE3) | pET28a(+) | Cellulase (CelA2) | Intracellular |
E. coli BL21-Gold (DE3) | pET22b(+) | Lipase A (BSLA) | Periplasmatic |
B. subtilis DB104 | pHY300PLK | Protease (subtilisin Carlsberg) | Extracellular |