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Table 1 Overview of the expression systems used for developing and validating the epMEGAWHOP method

From: Increasing protein production by directed vector backbone evolution

Expression host

Plasmid

Enzyme

Location of expressed enzyme

E. coli BL21-Gold (DE3)

pET28a(+)

Cellulase (CelA2)

Intracellular

E. coli BL21-Gold (DE3)

pET22b(+)

Lipase A (BSLA)

Periplasmatic

B. subtilis DB104

pHY300PLK

Protease (subtilisin Carlsberg)

Extracellular