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Table 1 Overview of the expression systems used for developing and validating the epMEGAWHOP method

From: Increasing protein production by directed vector backbone evolution

Expression host Plasmid Enzyme Location of expressed enzyme
E. coli BL21-Gold (DE3) pET28a(+) Cellulase (CelA2) Intracellular
E. coli BL21-Gold (DE3) pET22b(+) Lipase A (BSLA) Periplasmatic
B. subtilis DB104 pHY300PLK Protease (subtilisin Carlsberg) Extracellular