Figure 2From: Optimization of macroelement concentrations, pH and osmolarity for triacylglycerol accumulation in Rhodococcus opacus strain PD630 Growth of R. opacus under different nutrient concentrations and pH values. R. opacus was grown in minimal medium as described in the material and methods section. The varied conditions were A: ● 5 g l-1 sucrose, ○ 10 g l-1 sucrose, ▼ 20 g l-1 sucrose, Δ 30 g l-1 sucrose B: ● 40 g l-1 sucrose, ○ 120 g l-1 sucrose, ▼ 240 g l-1 sucrose C: ● 0.1 g l-1 ammonium, ○ 0.2 g l-1 ammonium, ▼ 0.4 g l-1 ammonium, Δ 0.7 g l-1 ammonium, ■ 1.0 g l-1 ammonium, □ 1.4 g l-1 ammonium D: ● 0.02 g l-1 magnesium, ○ 0.12 g l-1 magnesium, ▼ 0.51 g l-1 magnesium, Δ 1.01 g l-1 magnesium E: ● 3.4 g l-1 phosphate , ○ 6.8 g l-1 phosphate, ▼ 13.6 g l-1 phosphate. F: The cultivations were started at an initial pH value of ● 6.8, ○ 7.1, ▼ 7.4, Δ 7.7, ■ 8.0 G: Addition of NaCl to get an osmotic concentration of ● 230 mOsm l-1, ○ 450 mOsm l-1, ▼ 800 mOsm l-1 H: Addition of NaCl to reach an osmolarity of ● 1200 mOsm l-1, ○ 1600 mOsm l-1, ▼ 2000 mOsm l-1. Error bars represent the deviations of at least two independent cultivations.Back to article page