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Figure 2 | AMB Express

Figure 2

From: Optimization of macroelement concentrations, pH and osmolarity for triacylglycerol accumulation in Rhodococcus opacus strain PD630

Figure 2

Growth of R. opacus under different nutrient concentrations and pH values. R. opacus was grown in minimal medium as described in the material and methods section. The varied conditions were A: 5 g l-1 sucrose, 10 g l-1 sucrose, 20 g l-1 sucrose, Δ 30 g l-1 sucrose B: 40 g l-1 sucrose, 120 g l-1 sucrose, 240 g l-1 sucrose C: 0.1 g l-1 ammonium, 0.2 g l-1 ammonium, 0.4 g l-1 ammonium, Δ 0.7 g l-1 ammonium, 1.0 g l-1 ammonium, 1.4 g l-1 ammonium D: 0.02 g l-1 magnesium, 0.12 g l-1 magnesium, 0.51 g l-1 magnesium, Δ 1.01 g l-1 magnesium E: 3.4 g l-1 phosphate , 6.8 g l-1 phosphate, 13.6 g l-1 phosphate. F: The cultivations were started at an initial pH value of 6.8, 7.1, 7.4, Δ 7.7, 8.0 G: Addition of NaCl to get an osmotic concentration of 230 mOsm l-1, 450 mOsm l-1, 800 mOsm l-1H: Addition of NaCl to reach an osmolarity of 1200 mOsm l-1, 1600 mOsm l-1, 2000 mOsm l-1. Error bars represent the deviations of at least two independent cultivations.

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