Table 1 Characteristics of the E. coli and S. cerevisiae strains and plasmids used in this study
Strains and plasmids | Relevant features | Reference |
|---|---|---|
E. coli strain | ||
Novablue | endA1 hsdR17(r K12 - m K12 + ) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB+ lacIq Z∆M15::Tn10(Tetr)] | Novagen |
S. cerevisiae yeast strains | ||
MT8-1 | MATa ade leu2 his3 ura3 trp1 | Tajima et al. (1985) |
MT8-1/cocδBEC1 | MATa ade leu2 his3 ura3, Cocktail δ-integration of β-glucosidase, endoglucanase, and cellobiohydrolase genes | Yamada et al. (2010b) |
MT8-1/δCDTiBGL | MATa ade leu2 his3 trp1, Cocktail δ-integration of intracellular β-glucosidase and cellodextrin transporter genes | This study |
MT8-1/cocδBEC1/δCDTiBGL | MATa ade leu2, Cocktail δ-integration of β-glucosidase, endoglucanase, cellobiohydrolase, intracellular β-glucosidase, and cellodextrin transporter genes | This study |
Plasmids | ||
pδU-PGCDT | URA3, Expression of cellodextrin transporter by δ-integration | This study |
pδU-PGiBGL | URA3, Expression of intracellular β-glucosidase by δ-integration | This study |
pδH-PGiBGL | HIS3, Expression of intracellular β-glucosidase by δ-integration | This study |