Table 2 Amount of protein present in commercial preparations of common lipases
Commercial lipase preparation | Supplier | Protein content (%) | Method of measurement | Reference |
|---|---|---|---|---|
CRL (VII ) | Sigma Chemicals Co. (Missouri, USA) | 22 | n.a. | Arroyo (1995) |
CRL (VII) | Sigma Chemicals Co. (St. Louis, USA). | 6 | Lowry | López et al. (2004) |
CRL (VII) | Sigma Chemicals Co. (St. Louis, USA). | 9.3 | n.a. | Gitlesen et al. (1997) |
CRL (AY 30) | Amano (Nagoya, Japan) | 3.3 | Lowry | López et al. (2004) |
CRL (AY) | Amano (Nagoya, Japan) | 10 | n.a. | Gitlesen et al. (1997) |
CRL (AY) | Amano (Nagoya, Japan) | 1.1 | Bradford | Salis et al. (2008) |
PS (Lipase PS) | Amano (Nagoya, Japan) | 0,55 | Bradford | Salis et al. (2008) |
PS (Chirazyme L-9, Lyo) | Roche Diagnostics (Germany) | 12 | Micro-Bradford (Standard:BSA) | Guieysse et al. (2004) |
PFL (Lipase AY) | Amano (Nagoya, Japan) | 2 | Bradford | Salis et al. (2008) |
RML (Chirazyme L-9, Lyo) | Roche Diagnostics (Germany) | 15 | Micro-Bradford (Standard:BSA) | Guieysse et al. (2004) |
CALB Novozym 435 | Novozymes A/S (Bagsvaerd Denmark) | 8 | Precipitation with acetone | Prabhavathi et al. (2009) |
CALB SP525 | Novo Nordisk Biondustrias, S.A. (Madrid, España) | 10 | Biuret | López et al. (2004) |
Lipozyme CALB (Optimized value) | Novozymes | 30 | Precipitation with (NH4)2SO4 | This work |
Lipozyme TLL (Optimized value) | Novozymes | 20 | Precipitation with (NH4)2SO4 | This work |