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Table 2 Amount of protein present in commercial preparations of common lipases

From: Cross-linked enzyme aggregates (CLEAs) of selected lipases: a procedure for the proper calculation of their recovered activity

Commercial lipase preparation Supplier Protein content (%) Method of measurement Reference
CRL (VII ) Sigma Chemicals Co. (Missouri, USA) 22 n.a. Arroyo (1995)
CRL (VII) Sigma Chemicals Co. (St. Louis, USA). 6 Lowry López et al. (2004)
CRL (VII) Sigma Chemicals Co. (St. Louis, USA). 9.3 n.a. Gitlesen et al. (1997)
CRL (AY 30) Amano (Nagoya, Japan) 3.3 Lowry López et al. (2004)
CRL (AY) Amano (Nagoya, Japan) 10 n.a. Gitlesen et al. (1997)
CRL (AY) Amano (Nagoya, Japan) 1.1 Bradford Salis et al. (2008)
PS (Lipase PS) Amano (Nagoya, Japan) 0,55 Bradford Salis et al. (2008)
PS (Chirazyme L-9, Lyo) Roche Diagnostics (Germany) 12 Micro-Bradford (Standard:BSA) Guieysse et al. (2004)
PFL (Lipase AY) Amano (Nagoya, Japan) 2 Bradford Salis et al. (2008)
RML (Chirazyme L-9, Lyo) Roche Diagnostics (Germany) 15 Micro-Bradford (Standard:BSA) Guieysse et al. (2004)
CALB Novozym 435 Novozymes A/S (Bagsvaerd Denmark) 8 Precipitation with acetone Prabhavathi et al. (2009)
CALB SP525 Novo Nordisk Biondustrias, S.A. (Madrid, España) 10 Biuret López et al. (2004)
Lipozyme CALB (Optimized value) Novozymes 30 Precipitation with (NH4)2SO4 This work
Lipozyme TLL (Optimized value) Novozymes 20 Precipitation with (NH4)2SO4 This work