Table 1 Gene organization of bafilomycin biosynthetic gene cluster of Streptomyces griseus DSM 2608
ORF | Gene product | Number of amino acid | Deduced function | The closest gene | Gene strain | Homology (%) |
|---|---|---|---|---|---|---|
(GenBank number) | ||||||
ORF1 | BafBI | 460 | acyl-CoA ligase (partial) | ADC79613.1 | Streptomyces lohii | 93 |
ORF2 | BafBII | 513 | amide synthetase | ADC79614.1 | Streptomyces lohii | 96 |
ORF3 | BafBIII | 415 | 5-aminolevulinate synthase | ADC79615.1 | Streptomyces lohii | 98 |
ORF4 | BafSI | 5016 | modular polyketide synthase I | ADC79616.1 | Streptomyces lohii | 93 |
ORF5 | BafSII | 5089 | modular polyketide synthase II | YP_004909056.1 | Kitasatospora setae | 85 |
ORF6 | BafSIII | 3981 | modular polyketide synthase III | ADC79618.1 | Streptomyces lohii | 94 |
ORF7 | BafSIV | 3627 | modular polyketide synthase IV | ADC79619.1 | Streptomyces lohii | 89 |
ORF8 | BafSV | 2106 | modular polyketide synthase V | ADC79620.1 | Streptomyces lohii | 93 |
ORF9 | BafAI | 297 | 3-hydroxyacyl-CoA dehydrogenase (NAD-dependent) | ADC79621.1 | Streptomyces lohii | 97 |
ORF10 | BafAII | 94 | acyl carrier protein | ADC79622.1 | Streptomyces lohii | 97 |
ORF11 | BafAIII | 364 | acyl-CoA dehydrogenases (FAD-dependent) | ADC79623.1 | Streptomyces lohii | 98 |
ORF12 | BafAIV | 367 | FkbH-like protein | ADC79624.1 | Streptomyces lohii | 97 |
ORF13 | BafAV | 221 | O-methyltransferase | ADC79625.1 | Streptomyces lohii | 95 |
ORF14 | BafRI | 610 | AfsR family transcriptional regulator | ADC79626.1 | Streptomyces lohii | 96 |
ORF15 | BafT | 254 | Thioesterase II | ADC79627.1 | Streptomyces lohii | 95 |
ORF16 | BafRII | 118 | LuxR-like protein | ADC79628.1 | Streptomyces lohii | 91 |
ORF17 | BafCII | 321 | malonyl transferase | ADC79629.1 | Streptomyces lohii | 97 |
ORF18 | BafCI | 360 | Putative CoA ligase | ADC79630.1 | Streptomyces lohii | 97 |
ORF19 | Â | 511 | XRE family transcriptional regulator | ZP_04709597.1 | Streptomyces roseosporus | 98 |
ORF20 | Â | 506 | 2-methylcitrate reductase | ZP_04709598.1 | Streptomyces roseosporus | 95 |
ORF21 | Â | 379 | methylcitrate synthase | ZP_04709599.1 | Streptomyces roseosporus | 94 |
ORF22 | Â | 224 | GntR family transcriptional regulator | ZP_11438254.1 | Mycobacterium abscessus | 53 |
ORF23 | Â | 285 | methylcitrate lyase | ZP_04709600.1 | Streptomyces roseosporus | 91 |
ORF24 | Â | 491 | putative transcriptional regulator | ZP_19189491.1 | Streptomyces ipomoeae | 71 |
ORF25 | Â | 846 | Osmosensitive K+ channel histidine kinase | ZP_04995839.1 | Streptomyces sp. Mg1 | 84 |
ORF26 | Â | 225 | K+-transporting ATPase subunit C | ZP_04710866.1 | Streptomyces roseosporus | 82 |
ORF27 | Â | 486 | K+-transporting ATPase subunit B (partial) | YP_001824190.1 | Streptomyces griseus subsp. griseus | 91 |