Figure 1

Organization of bafilomycin biosynthetic gene cluster. The overall gene cluster was made by assembly of pSGB-1, pSGB-20 and pSGB-23 cosmids. The upstream region was further amplified by using ACL and ALA primers. The total length of the cloned gene cluster was 87,324 bp, which was deposited in GenBank under accession number KC514471. In this gene cluster, 27 ORFs including 5 modular polyketide synthase genes were found, as described in TableĀ 1.