Skip to main content

Table 3 Specific activities and ranking of lipases in P(3HB- co -92 mol% 4HB), p NPL and olive oil hydrolysis assay

From: Densitometry based microassay for the determination of lipase depolymerizing activity on polyhydroxyalkanoate

Sources of lipases P(3HB- co-92 mol% 4HB) p NPL   Olive oil  
  Specific activity (Relative density/ min/ mg) Rank* Specific activity (μmol 4-nitrophenol/ min/ mg) Rank† Specific activity (μmol fatty acid/ min/ mg) Rank #
C. viscosum 4369.47 1 9800 1 365.00 1
C. antarctica 596.29 2 44 4 0.60 11
M. miehei 249.67 3 8.1 6 49.35 2
P. cepacia 58.76 4 80 2 15.28 3
R. oryzae 52.8 5 0.1 10 6.19 6
R. arrhizus 45.52 6 6.2 7 10.69 4
Porcine pancreas 32.19 7 0.25 8 0.90 10
P. fluorescens 23.97 8 37 5 5.64 7
M. javanicus 21.05 9 0.15 9 2.83 8
C. rugosa 15.21 10 68 3 8.72 5
R. niveus 1.06 11 0.03 11 1.53 9
  1. * Rank according to specific activity by using P(3HB-co-4HB) as substrate, †Rank according to specific activity by using p-NPL as substrate, #Rank according to specific activity by using olive oil as substrate.
\