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Table 1 List of commercial lipases used in the current study (Sigma-Aldrich technical information)

From: Densitometry based microassay for the determination of lipase depolymerizing activity on polyhydroxyalkanoate

Lipase name Product number Lipase activity (U/mg) Unit definition
Lipase from Aspergillus oryzae 62285 ~50 1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Fluka No. 62314 as substrate)
Amano Lipase A from Aspergillus niger 534781 ≥12 -
Lipase from Candida antarctica 65986 ≥1.0 1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Fluka No. 62314 as substrate)
Lipase from Candida rugosa, Type VII L1754 ≥700 1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37°C
Amano Lipase M from Mucor javanicus 534803 ≥10 -
Lipase from Mucor miehei L9031 ≥4,000 1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37°C using olive oil
Lipase from Rhizopus arrhizus 62305 ~10 1 U corresponds to the amount of enzyme which liberates 1 μmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Fluka No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Fluka No. 62314 as substrate, at pH 8.0 and 40°C
Lipase from Rhizopus niveus 62310 ≥1.5 1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Fluka No. 62314, at pH 8.0 and 40°C as substrate
Lipase from Rhizopus oryzae 80612 ≥30 1 U corresponds to the amount of enzyme which releases 1 μmol fatty acid from triglycerides per minute at pH 7.2 and 37 C (olive oil as substrate)
Lipase from Chromobacterium viscosum, Type XII L0763 ≥2,000 1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37°C using olive oil (30 minute incubation)
Lipase from Pseudomonas cepacia 62309 ≥30 1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Fluka No. 62314 as substrate)
Amano Lipase from Pseudomonas fluorescens 534730 ≥20 -
Lipase from porcine pancreas, Type II L3126 100-400 (using olive oil, 30 min incubation), 30–90 (using triacetin) 1 U will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
Lipase from wheat germ, Type I L3001 5-15 1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37°C
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