Table 1 List of commercial lipases used in the current study (Sigma-Aldrich technical information)

Lipase from Aspergillus oryzae

62285

~50

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Fluka No. 62314 as substrate)

Amano Lipase A from Aspergillus niger

534781

≥12

-

Lipase from Candida antarctica

65986

≥1.0

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Fluka No. 62314 as substrate)

Lipase from Candida rugosa, Type VII

L1754

≥700

1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37°C

Amano Lipase M from Mucor javanicus

534803

≥10

-

Lipase from Mucor miehei

L9031

≥4,000

1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37°C using olive oil

Lipase from Rhizopus arrhizus

62305

~10

1 U corresponds to the amount of enzyme which liberates 1 μmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Fluka No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Fluka No. 62314 as substrate, at pH 8.0 and 40°C

Lipase from Rhizopus niveus

62310

≥1.5

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Fluka No. 62314, at pH 8.0 and 40°C as substrate

Lipase from Rhizopus oryzae

80612

≥30

1 U corresponds to the amount of enzyme which releases 1 μmol fatty acid from triglycerides per minute at pH 7.2 and 37 C (olive oil as substrate)

Lipase from Chromobacterium viscosum, Type XII

L0763

≥2,000

1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37°C using olive oil (30 minute incubation)

Lipase from Pseudomonas cepacia

62309

≥30

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Fluka No. 62314 as substrate)

Amano Lipase from Pseudomonas fluorescens

534730

≥20

-

Lipase from porcine pancreas, Type II

L3126

100-400 (using olive oil, 30 min incubation), 30–90 (using triacetin)

1 U will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Lipase from wheat germ, Type I

L3001

5-15

1 U will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37°C