Table 1 Primers used for reverse transcription (RT)-PCR and real-time (qRT)-PCR
Name | Primer sequence |
|---|---|
Reverse transcription PCR | Â |
glnA-for | 5′-GGCTTCTGCTCGGTGATCTT-3′ |
glnA-rev | 5′-GACTGGGCCAGCTTATCCAC-3′ |
glnB-for | 5′-GTCCTTGCGAGGGTATCGAG-3′ |
glnB-rev | 5′-CGACCAGTCAGACGCTCATC-3′ |
gdh1-for | 5′-TCCGACGATCTCAATGTTGG-3′ |
gdh1-rev | 5′-CGCCGTCTACATCGATACCA-3′ |
gdh2-for | 5′-AAAGTGAATATGGGTGGCGG-3′ |
gdh2-rev | 5′-TCCAGTTGCTTCAGGTCGAA-3′ |
Real-time PCR | Â |
glnB-RT-for | 5′-GAGGGTATCGAGATGGGTGA-3′ |
glnB-RT-rev | 5′-GGTGGAAGGAGATCTGGACA-3′ |
gdh1-RT-for | 5′-GTGTTACCTCGCTAGACGGC-3′ |
gdh1-RT-rev | 5′-TCTACTCGATAGCTTCGCCC- 3′ |