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Table 2 Yields and kinetic parameters obtained during chemostat cultivations

From: Specific growth rate and substrate dependent polyhydroxybutyrate production in Saccharomyces cerevisiae

Feeding component

Dilution rate

Y sx

Y sEtOH

Y sPHB

Ethanol accumulation

Y xPHB

h -1

Cmol/Cmol

Cmol/Cmol

Cmmol/Cmol

Cmol/L

mg/gDW

Glucose

0.05

0.51 ± 0.01

-

2.51 ± 0.07

0

4.33 ± 0.19

0.10

0.57 ± 0

-

3.67 ± 0

0

5.59 ± 0

0.15

0.36 ± 0.02

0.02 ± 0

2.44 ± 0

-

5.94 ± 0.64

0.20

0.29 ± 0

0.1 ± 0

1.92 ± 0

-

5.59 ± 0.23

Ethanol

0.05

0.45 ± 0.01

0

8.50 ± 0.23

0

16.55 ± 0.02

0.10

0.37 ± 0

-

4.94 ± 0.58

0.0964 ± 0

13.49 ± 0

0.15

0.12 ± 0.01

-

0.46 ± 0.05

0.2364 ± 0

5.30 ± 0.31

Glucose: Ethanol (1:2)

0.05

0.48 ± 0.02

0

9.97 ± 0.07

0

18.34 ± 0.53

0.10

0.38 ± 0

-

7.41 ± 0

0.2012

12.41 ± 0

0.15

0.30 ± 0

-

2.57 ± 0.1

0.1637

7.50 ± 0.19

0.20

0.22 ± 0

-

1.13 ± 0

0.2769

4.56 ± 0

  1. The values were calculated from duplicate fermentations and are represented as mean ± SD. The formula for biomass used in this calculation is CH1.8O0.5 N0.2. Ysx = biomass yield on substrate, YsEtOH = ethanol yield on substrate, YsPHB = PHB yield on substrate, YxPHB = PHB yield per biomass.