Table 2 Effect of various compounds on the activity of the purified peroxidase, the results are shown as a mean of remained activity ± standard deviation of three replicates
Compounds | Concentration | % Relative activity |
|---|---|---|
EDTA | 1 mM | 85.43±11.48 |
5 mM | 91.05±8.58 | |
10 mM | 69.06±10.01 | |
β-mercaptoethanol | 1 mM | 82.98±4.76 |
5 mM | 0.00 | |
10 mM | 0.00 | |
NaN3 | 1 mM | 45.94±8.98 |
5 mM | 25.18±4.90 | |
10 mM | 18.99±3.31 | |
SDS | 0.5% | 84.75±5.20 |
2.0% | 86.51±4.69 | |
4.0% | 89.16±4.83 |