Table 1 Summary of the purification of the peroxidase isolated from a H. brasiliensis cell suspension
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification factor | Yield (%) |
|---|---|---|---|---|---|
Crude | 10.810 | 18796.46 | 1738.75 | 1 | 100.00 |
DEAE | 0.213 | 797.95 | 3752.97 | 2.16 | 4.25 |
ConA-agarose | 0.061 | 355.25 | 5820.53 | 3.35 | 1.89 |
Prep-SDS-PAGE | 0.004 | 331.88 | 76872.67 | 44.21 | 1.77 |