Figure 2From: Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans Correlation between the level of transcription of the aphII gene and the survival rates of different transformants in the presence of 100 μg.ml-1 neomycin. (A) Assessment of the transcriptional activity of aphII in S. Lividans driven by the promoters highlighted in bold in Figure 1 and the control plasmids, pIJ487 and pIJ487ermE* , using RT-PCR. Clones were ordered from the left to the right according to increasing promoter strength. (B) Quantification of the RT-PCR signals was made using ImageQuant pixel counts in non-saturated conditions. Values were normalized on pIJ487 signals. Survival rates corresponding to each clone are indicated above each histogram. (C) Plotting of the quantification of the RT-PCR products of the different clones versus their corresponding survival rates in presence of 100 μg.ml-1 neomycin.Back to article page