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Table 2 Relative productivities of SELP-1020-A as determined by area calculation of protein bands

From: High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures

  Relative percentage (%)
  LB+lac 37°C TB+lac 37°C TB+lac 30°C Flask volume ratios (TB+lac, 37°C)
     1:2 1:3 1:4 1:5
15 hours n.d. 89 n.d. n.d. n.d. n.d. n.d.
16 hours 86 87 37 n.d. n.d. n.d. n.d.
18 hours 100 100 50 n.d. n.d. n.d. n.d.
20 hours 99 96 67 n.d. n.d. n.d. n.d.
22 hours 93 99 91 100 99 100 98
24 hours 95 97 100 n.d. n.d. n.d. n.d.
  1. Bacterial cells were cultured in LB+lac at 37°C, TB+lac at 37°C, TB+lac at 30°C and TB+lac at 37°C with different liquid to flask volume ratios (1:2, 1:3, 1:4 and 1:5). Band areas were calculated with ImageJ and expressed as relative percentage. n.d. – not determined.