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AMB Express

Table 2 Relative productivities of SELP-1020-A as determined by area calculation of protein bands

From: High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures

 

Relative percentage (%)

 

LB+lac 37°C

TB+lac 37°C

TB+lac 30°C

Flask volume ratios (TB+lac, 37°C)

    

1:2

1:3

1:4

1:5

15 hours

n.d.

89

n.d.

n.d.

n.d.

n.d.

n.d.

16 hours

86

87

37

n.d.

n.d.

n.d.

n.d.

18 hours

100

100

50

n.d.

n.d.

n.d.

n.d.

20 hours

99

96

67

n.d.

n.d.

n.d.

n.d.

22 hours

93

99

91

100

99

100

98

24 hours

95

97

100

n.d.

n.d.

n.d.

n.d.

  1. Bacterial cells were cultured in LB+lac at 37°C, TB+lac at 37°C, TB+lac at 30°C and TB+lac at 37°C with different liquid to flask volume ratios (1:2, 1:3, 1:4 and 1:5). Band areas were calculated with ImageJ and expressed as relative percentage. n.d. – not determined.
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