Evaluation of cell death and protein release in bacterial cultures of SELP-59-A. (A) – number of CFUs in untreated cell crude extracts (U) and in cell extracts submitted to sonication (S), acid-based cell lysis (Ac) and acid-based cell lysis followed by sonication (AcS). Cell dilutions are represented in parenthesis. (B) – Graphic representation of the percentage of cell survival for the different treatments with results expressed as percentage of survival as compared to the untreated sample. (C) – Evaluation of protein release in samples at different concentrations of wet cell weight after acid-based cell lysis (Ac) and acid-based cell lysis followed by sonication (AcS). The same volume was applied in each lane. Samples legend: U – untreated cell crude extract; Ac – acid-based cell lysis; AcS – acid-based cell lysis followed by sonication. (D) – FTIR spectra displaying the amide I (1600 – 1700 cm-1) and amide II (1500 – 1600 cm-1) band regions of pure lyophilized SELP-59-A samples without (a) and with (b) the acidic treatment.