Skip to main content

Table 2 The specific activities of NADH oxidase and NADH peroxidase of L. panis PM1

From: Influence of oxygen on NADH recycling and oxidative stress resistance systems in Lactobacillus panis PM1

Enzyme sources a Enzyme assay conditions Enzyme activity b Relative gene expression level j
NADH oxidase NADH peroxidase nox npx
Aerobic culture Aerated condition 158.8 ± 7.6 d 148.3 ± 9.7 h 1.50 ± 0.30 1.16 ± 0.28
Microaerobic culture 144.0 ± 2.0 e N.D c 1.00 ± 0.22 1.00 ± 0.10
Aerobic culture Non-aerated condition 92.5 ± 2.2 f 197.3 ± 1.7 i   
Microaerobic culture 103.1 ± 5.6 g N.D c   
  1. a . Lactobacillus panis PM1 was cultured in mMRS for 24 hours under aerobic or microaerobic conditions for enzyme assays. b. Values represent specific activities (unit/mg protein). One unit is defined as one micromole of H2O2 generated (for NADH oxidase) or decomposed (for NADH peroxidase) per minute. c. Minus values where H2O2 generating ability was higher than H2O2 decomposing ability are defined as non-detectable (N.D) in NADH peroxidase assay. d. Significantly different to f, P < 0.05. e. Significantly different to g, P < 0.05. h. Significantly different to i, P < 0.05. j. Microaerobic culture was used for control sample for relative gene expression by qRT-PCR.