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Figure 3 | AMB Express

Figure 3

From: Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide

Figure 3

Use of PMA on multispecies biofilms. Oral multispecies biofilm comprising by S. oralis, S. gordonii, V. parvula, F. nucleatum and P. intermedia were exposed to CPC and treated with 100 μM PMA. The number of dead cells (Δlog10 cells) was found by calculating the difference between the amount of total cells (qPCR) and the amount of live cells (qPCR-PMA). Bars indicate the mean values for each species in a biofilm not exposed to CPC (empty bars) and a CPC-killed biofilm (grey bars).

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