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Table 1 Degenerate primers used for the searching for partial CYP cDNAs from P. brevispora

From: Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora

Target gene Forward primer Reverse primer
pb- 1 (first PCR) 5'(A/C/T)TIIIIG(C/G)IGGI(A/C/G)(A/G)I(C/G)AIACIACIGC-3' 5'-CCI(A/G)(A/G/T)(A/G)CAIIII(C/T)(G/T)II(C/G/T)ICCII(A/C/G)I (C/T)(C/T)(A/G)AAIGG-3'
pb- 1 (second PCR) 5'-T(G/T)(C/G/T)(A/C/G)(A/G)IC CI GAI(A/C)GIT(G/T)(C/G/T) (C/G/T)T-3' 5'-(C/T)(G/T)IIIICCII(A/C/G)I(C/T)(C/T)(A/G)AAIGGIA(A/G/T)I T(A/G)-3'
pb- 2, pb- 3 5'-(C/T)TIAA(C/T)ATI(A/C/T)TI(A/C/T)TIGCIGGI(A/C)GIGA(C/T)ACIAC-3' 5'-(C/T)TGICCIA(A/G)(A/G)CA IA(A/G/T)IC(G/T)IGGICCIGC-3'