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Table 2 Constructed plasmids for membrane display of somatostatin in this study

From: Membrane-displayed somatostatin activates somatostatin receptor subtype-2 heterologously produced in Saccharomyces cerevisiae

Plasmid Description
pYSn-sst (n = 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 20, 21, 22, 30, 40) Membrane display of somatostatin with FLAG tag and various linker lengths
pYS0-nega Membrane display of FLAG tag (same construct as pYS0-sst but lack somatostatin sequence, and used as a negative control)
pYS-notag3-sst, pYS-notag20-sst, pYS-notag38-sst Membrane display of somatostatin without FLAG tag
pYS21-sst(W8A), pYS21-sst(C14A) Membrane display of alanine-substituted somatostatin with FLAG tag