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Table 2 Evaluation of PCR inhibition via bacterial 16S rRNA gene amplification in undiluted (1x) versus diluted DNA extracts a

From: Automated DNA extraction platforms offer solutions to challenges of assessing microbial biofouling in oil production facilities

 

1xb

1:10xb

1:100xc

Sample A

   

P

2.03±0.15x109

2.07±0.02x109

1.32±0.07x109

Q

6.26±0.57x109

6.06±0.13x109

2.88±0.04x109

M

2.55±0.26x109

2.38±0.10x109

2.46±0.08x109

Sample B

   

P

8.44±0.17x108

7.33±0.10x108

5.79±0.10x108

Q

6.34±0.56x109

6.32±0.13x109

3.72±0.19x109

M

4.70±0.08x109

5.16±0.20x109

3.95±0.23x109

Sample C

   

P

6.28±0.43x106

6.83±0.85x106

7.48±0.52x106

Q

9.26±5.48x106

9.59±0.28x106

8.83±0.18x106

M

1.74±0.04x108

1.14±0.05x108

1.15±0.01x108

  1. aData shown are mean values (n = 3) and standard deviations of 16S rRNA gene copies per ml original sample.
  2. bStandard curve: m = −3.67; y-int = 41.61; R2 = 0.99; Eff = 87.1%.
  3. cStandard curve: m = −3.59; y-int = 41.15; R2 = 0.99; Eff = 89.7%.