|
Platform
|
PowerBiofilm
|
QuickGene
|
Maxwell
|
|---|
|
Ease of operation
|
Manual
|
Semi-automated
|
Automated
|
|
Dedicated instrument
|
No
|
QuickGene-Mini80
|
Maxwell 16
|
|
Costa
| |
$1500
|
$25000
|
|
Processing steps
| | | |
|
Sample preparation
|
Manual
|
Manual
|
Manual
|
|
Cell lysis
|
Manual
|
Manual
|
Automated
|
|
Inhibitor removal
|
Manual
|
Manual
|
Automated
|
|
Nucleic acid mobilization
|
Manual
|
Automated
|
Automated
|
|
3 x Washes
|
Manual
|
Automated
|
Automated
|
|
Elution
|
Manual
|
Automated
|
Automated
|
|
Total time
|
120 min (n = 10)
|
60 min (n = 8)
|
45 min (n = 16)
|
|
Additional equipment
| | | |
|
Microcentrifuge
|
Yes
|
Yes
|
No
|
|
Incubator (>55°C)
|
Yes
|
Yes
|
No
|
|
Physical lysis equipmentb
|
Yes
|
Yes
|
No
|
|
Refrigerator (4°C)
|
Yes
|
No
|
No
|
|
Consumable supplies
| | | |
|
Cost (per sample)
|
$6.50
|
$2.60
|
$6.33
|
- aApproximate list price at time of purchase.
- bPhysical lysis equipment: a bead-beater for PowerBiofilm and a rotisserie for Quickgene.
