Table 1 Comparison of DNA extraction platforms
Platform | PowerBiofilm | QuickGene | Maxwell |
|---|---|---|---|
Ease of operation | Manual | Semi-automated | Automated |
Dedicated instrument | No | QuickGene-Mini80 | Maxwell 16 |
Costa | $1500 | $25000 | |
Processing steps | |||
Sample preparation | Manual | Manual | Manual |
Cell lysis | Manual | Manual | Automated |
Inhibitor removal | Manual | Manual | Automated |
Nucleic acid mobilization | Manual | Automated | Automated |
3 x Washes | Manual | Automated | Automated |
Elution | Manual | Automated | Automated |
Total time | 120 min (n = 10) | 60 min (n = 8) | 45 min (n = 16) |
Additional equipment | |||
Microcentrifuge | Yes | Yes | No |
Incubator (>55°C) | Yes | Yes | No |
Physical lysis equipmentb | Yes | Yes | No |
Refrigerator (4°C) | Yes | No | No |
Consumable supplies | |||
Cost (per sample) | $6.50 | $2.60 | $6.33 |